IMP-4974

TGFBR2

Recombinant extracellular domain (Met 1-Asp 159) of human TGFβ receptor 2 (NP_003233.4) was expressed with the fused C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

TGFBR2 is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised of a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilized by a network of six intra strand disulfide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity.

HEK293

Human

C-His&C-hFc

Ile 24

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human TGFβ R2/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The monomer consists of 383 amino acids and predicts a molecular mass of 43.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of this monomer is approximately 60-65 kDa due to the glycosylation.

Met1-Asp159

< 1.0 EU/μg of the protein as determined by the LAL method

> 97 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Measured by its binding ability in a functional ELISA. Immobilized TGFBR2h (1-166Q) at 10 μg/mL (100 μL/well) can bind TGFB1-His/Biotin, the EC50 of human TGFB1-His/Biotin is 120-240 ng/mL.