Recombinant Human TGFBR2 Protein (Thr23-Asp184 & Ile24-Asp184), C-Fc-tagged
Product Description
Cat
IMP-9550
Official Symbol
TGFBR2
Product Overview
Recombinant human TGF- beta RII Isoform 2 (Thr23-Asp184) & (Ile24-Asp184) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of genes related to cell proliferation, cell cycle arrest, wound healing, immunosuppression, and tumorigenesis. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-Fc
Predicted N Terminal
Thr23 & Ile24
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 45 kDa (monomer)
SDS-PAGE: 65-75 kDa, reducing conditions
Protein length
Thr23-Asp184 & Ile24-Asp184
Bio-activity
Measured by its ability to inhibit TGF-beta 1 activity on HT-2 mouse T cells. The ED50 for this effect is 1-3 ng/mL in the presence of 0.1 ng/mL of recombinant human TGF-beta 1.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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