Recombinant Human THBS1 Protein (Asn19-Pro1170, Thr523Ala), C-10×His-tagged

Product Description

Cat

IMP-9751

Official Symbol

THBS1

Product Overview

Recombinant human Thrombospondin-1 protein (Asn19-Pro1170, Thr523Ala) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Asn19

Form

Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.

Molecular Mass

Predicted Molecular Mass: 129 kDa
SDS-PAGE: 135-165 kDa, reducing conditions

Protein length

Asn19-Pro1170, Thr523Ala

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF is coated at 1.00 μg/mL (100 μL/well), it binds to Recombinant Human Thrombospondin-1. The ED50 for this binding is 100-600 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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