Recombinant Human THBS1 Protein (Asn19-Pro1170, Thr523Ala), C-10×His-tagged

Product Description

Cat

IMP-9751

Official Symbol

THBS1

Product Overview

Recombinant human Thrombospondin-1 protein (Asn19-Pro1170, Thr523Ala) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Asn19

Form

Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.

Molecular Mass

Predicted Molecular Mass: 129 kDa
SDS-PAGE: 135-165 kDa, reducing conditions

Protein length

Asn19-Pro1170, Thr523Ala

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF is coated at 1.00 μg/mL (100 μL/well), it binds to Recombinant Human Thrombospondin-1. The ED50 for this binding is 100-600 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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