Recombinant Human THBS2 Protein (Gly19-Ile1172), C-10×His-tagged
Product Description
Cat
IMP-9696
Official Symbol
THBS2
Product Overview
Recombinant human Thrombospondin-2 protein (Gly19-Ile1172) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-10×His
Predicted N Terminal
Gly19
Form
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Molecular Mass
Predicted Molecular Mass: 129 kDa
SDS-PAGE: 170-176 kDa, reducing conditions
Protein length
Gly19-Ile1172
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 0.07-0.7 μg/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Bioactivity-ELISA 1

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 0.07-0.7 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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