Recombinant Human TIGIT protein, C-Avi-His Tag, Biotinylated

Product Description

Cat

IMP-039

Official Symbol

TIGIT

Product Overview

Biotinylated Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1-1) is expressed from human 293 cells (HEK293). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.

Expression System

HEK293

Species

Human

Tag

C-Avi-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 16.8 kDa. The protein migrates as 18-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Met 22-Pro 141

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Biotinylated Human TIGIT, Avitag,His Tag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Anti-Human TIGIT MAb at 2 ug/mL (100 uL/well) can bind Biotinylated Human TIGIT, Avitag,His Tag (recommended for biopanning) with a linear range of 0.2-3 ng/mL.

Bioactivity-FACS 1

FACS assay shows Biotinylated Human TIGIT, Avitag,His Tag (recommended for biopanning) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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