Recombinant Human TIGIT protein, C-Fc Tag
Product Description
Cat
IMP-002
Official Symbol
TIGIT
Product Overview
Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1 -1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Met 22-Pro 141
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human CD155, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human TIGIT, Fc Tag with a linear range of 0.6-5 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Human TIGIT, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag with a linear range of 0.313-10 ug/mL.

Bioactivity-ELISA 3
Bioactivity-ELISA

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag: Biotinylated Human CD155, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 ug/mL.

Bioactivity-BLI 1

Loaded Human TIGIT, Fc Tag on Protein A Biosensor, can bind Human CD155, His Tag with an affinity constant of 0.98 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1
Bioactivity-FACS

FACS assay shows that recombinant Human TIGIT, Fc Tag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 ug/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
Address

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies
x