Recombinant Human TIGIT protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-038

Official Symbol

TIGIT

Product Overview

Biotinylated Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1-1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 85-115 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Met 22-Pro 141

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Biotinylated Human TIGIT, Fc,Avitag (MALS verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Biotinylated Human TIGIT, Fc,Avitag (MALS verified) was more than 85% and around 95-115 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human CD155, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human TIGIT, Fc,Avitag with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag, low endotoxin: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Fc Tag: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.06065 ug/mL.

Bioactivity-FACS 1

FACS assay shows Biotinylated Human TIGIT, Fc,Avitag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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