Recombinant Human TIGIT protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-038

Official Symbol

TIGIT

Product Overview

Biotinylated Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1-1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 85-115 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Met 22-Pro 141

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Biotinylated Human TIGIT, Fc,Avitag (MALS verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Biotinylated Human TIGIT, Fc,Avitag (MALS verified) was more than 85% and around 95-115 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human CD155, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human TIGIT, Fc,Avitag with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag, low endotoxin: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Fc Tag: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.06065 ug/mL.

Bioactivity-FACS 1

FACS assay shows Biotinylated Human TIGIT, Fc,Avitag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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