Recombinant Human TIGIT protein, C-His Tag

Product Description

Cat

IMP-040

Official Symbol

TIGIT

Product Overview

Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Met 22-Pro 141

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human TIGIT, His Tag (MALS verified) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human TIGIT, His Tag at 2 ug/mL (100 uL/well) can bind Human CD155, Fc Tag with a linear range of 8-128 ng/mL.

Bioactivity-ELISA 2

Immobilized Human TIGIT, His Tag at 1 ug/mL (100 uL/well) can bind Human Nectin-3, Fc Tag with a linear range of 0.2-2.5 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 ug/mL to 0.0097 ug/mL) were added into Human TIGIT, His Tag: Biotinylated Human CD155, Fc Tag binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Bioactivity-BLI 1

Loaded Anti-Human TIGIT MAb (Mouse IgG1) on AMC Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 3.72 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CD155, Fc Tag on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.23 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human Nectin-3, Fc Tag on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.732 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human TIGIT, His Tag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.3 ug/mL.

Bioactivity-SPR 1

Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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