Recombinant Human TIGIT protein, C-His Tag

Product Description

Cat

IMP-040

Official Symbol

TIGIT

Product Overview

Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Met 22-Pro 141

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human TIGIT, His Tag (MALS verified) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human TIGIT, His Tag at 2 ug/mL (100 uL/well) can bind Human CD155, Fc Tag with a linear range of 8-128 ng/mL.

Bioactivity-ELISA 2

Immobilized Human TIGIT, His Tag at 1 ug/mL (100 uL/well) can bind Human Nectin-3, Fc Tag with a linear range of 0.2-2.5 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 ug/mL to 0.0097 ug/mL) were added into Human TIGIT, His Tag: Biotinylated Human CD155, Fc Tag binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Bioactivity-BLI 1

Loaded Anti-Human TIGIT MAb (Mouse IgG1) on AMC Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 3.72 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CD155, Fc Tag on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.23 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human Nectin-3, Fc Tag on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.732 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human TIGIT, His Tag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.3 ug/mL.

Bioactivity-SPR 1

Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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