Recombinant Human TIGIT Protein, C-His-tagged, Biotinylated

Product Description

Cat

IMP-1365

Official Symbol

TIGIT

Product Overview

Recombinant TIGIT (NP_776160.2) (Met1-Phe138) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Description

TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Met 22

Form

Lyophilized from sterile Sterile PBS, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant TIGIT consists of 128 amino acids and predicts a molecular mass of 14.2 kDa.

Protein length

Met1-Phe138

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT His, Biotinylated at 2 μg/mL (100 μL/well) can bind Mouse CD155/PVR His&hFc, the EC50 of Mouse CD155/PVR His&hFc is 50-250 ng/mL.

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of TIGIT Protein, Human, Recombinant (His Tag), Biotinylated bound to MITG7192A was 0.1 nM.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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