Recombinant Human TIGIT protein, C-mFc Tag

Product Description

Cat

IMP-037

Official Symbol

TIGIT

Product Overview

Recombinant Human TIGIT protein(AA Met 22-Pro 141)(Accession # Q495A1-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Met 22-Pro 141

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human CD155, Fc Tag at 5 ug/mL (100 uL/well) can bind Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.5-8 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 ug/mL to 0.0097 ug/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag: Biotinylated Human Nectin-2, Fc,Avitag binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Bioactivity-BLI 1

Loaded Human TIGIT, Mouse IgG2a Fc Tag on Protein A Biosensor, can bind Human CD155, His Tag with an affinity constant of 0.92 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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