Recombinant Human TNF protein, C-Fc Tag

Product Description

Cat

IMP-614

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(NP_000585.2)(Leu 25-Ser 645), fused to C-Fc Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Ser 645

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Human EGF, Tag Free at 10 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag with a linear range of 0.078-2.5 μg/mL.

Bioactivity-ELISA 2

Immobilized Human EGF R, Fc Tag at 1 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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