Recombinant Human TNF protein, C-His Tag, FITC-Labeled

Product Description

Cat

IMP-617

Official Symbol

TNF

Product Overview

FITC-Labeled Recombinant Human TNF protein(NP_000585.2)(Leu 25-Ser 645), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag, FITC-Labeled

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 70.5 kDa. The protein migrates as 85-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Ser 645

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human EGF R, His Tag with a linear range of 0.039-0.313 μg/mL.

Bioactivity-FACS 1

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Human EGF R Protein, His Tag and negative control protein respectively, FITC signal was used to evaluate the binding activity.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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