Recombinant Human TNF protein, C-His Tag
Product Description
Cat
IMP-5941
Official Symbol
TNF
Product Overview
Recombinant Human TNF protein(P01375)(77-233aa), fused with C-terminal His tag, was expressed in E.coli.
Expression System
E.coli
Species
Human
Tag
C-His Tag
Form
Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4
Molecular Mass
18.5 kDa
Protein length
77-233aa
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Biological Activity
The ED50 as determined in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D is less than 0.1 ng/ml.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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