Recombinant Human TNF Protein, C-His-tagged
Product Description
Cat
IMP-8955
Official Symbol
TNF
Product Overview
Human TNF recombinant protein with polyhistidine tag at the C-terminus expressed in E. coli.
Description
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Expression System
E. coli
Species
Human
Tag
C-His
Form
Lyophilized from a solution containing 1X PBS, pH 8.0.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.Please use within one month after protein reconstitution.
AA Sequence
MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Endotoxin
< 0.1 EU/μg of the protein by the LAL method.
Purity
> 98% by SDS-PAGE
Applications
Functional Study; SDS-PAGE
Storage
Store at -20 centigrade, lyophilized protein is stable for 1 year. After reconstitution with deionized water, store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.
SDS-PAGE
Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

Bioactivity-ELISA 1
Functional Study

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <0.2 pg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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