Recombinant Human TNF protein
Product Description
Cat
IMP-5877
Official Symbol
TNF
Product Overview
Recombinant Human TNF protein(P01375)(87-233aa) was expressed in E.Coli.
Expression System
E.Coli
Species
Human
Tag
N/A
Form
Lyophilized from a 0.2 μm filtered PBS, pH 7.0
Molecular Mass
16.9 kDa
Protein length
87-233aa
AA Sequence
MRKR+KPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Purity
>98% as determined by SDS-PAGE and HPLC.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 10^7 IU/mg in the presence of actinomycin D.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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