Recombinant Human TNF protein, N-His Tag

Product Description

Cat

IMP-5876

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(P01375)(77-233aa), fused with N-terminal His tag, was expressed in E.Coli.

Expression System

E.Coli

Species

Human

Tag

N-His Tag

Form

Lyophilized from a 0.2 m filtered PBS, pH 7.0

Molecular Mass

18.3 kDa

Protein length

77-233aa

AA Sequence

MHHHHHH+VRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL

Endotoxin

Less than 1.0 EU/µg as determined by LAL method.

Purity

>97% as determined by SDS-PAGE and HPLC.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of ＞ 2.0 × 107 IU/mg in the presence of actinomycin D.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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