Recombinant Human TNF protein, N-His Tag

Product Description

Cat

IMP-5942

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(P01375)(57-233aa), fused with N-terminal His tag, was expressed in E.coli.

Expression System

E.coli

Species

Human

Tag

N-His Tag

Form

Lyophilized from a 0.2 μm filtered 20 mM PB, 100 mM NaCl, pH 7.2

Molecular Mass

21.8 kDa

Protein length

57-233aa

AA Sequence

GPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Endotoxin

Less than 1.0 EU/µg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity

The ED50 as determined in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D is less than 1 ng/ml.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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