Recombinant Human TNF protein, N-His Tag

Product Description

Cat

IMP-5994

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(P01375)(77-233aa), fused with N-terminal His tag, was expressed in Yeast.

Expression System

Yeast

Species

Human

Tag

N-His Tag

Form

Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Molecular Mass

19.4 kDa

Protein length

77-233aa

AA Sequence

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Endotoxin

Not test.

Purity

Greater than 90% as determined by SDS-PAGE.

Biological Activity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ug/ml can bind human TNFRSF1B, the EC50 is 1.632-2.699 ng/ml

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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