Recombinant Human TNF protein, N-His Tag

Product Description

Cat

IMP-5994

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(P01375)(77-233aa), fused with N-terminal His tag, was expressed in Yeast.

Expression System

Yeast

Species

Human

Tag

N-His Tag

Form

Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Molecular Mass

19.4 kDa

Protein length

77-233aa

AA Sequence

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Endotoxin

Not test.

Purity

Greater than 90% as determined by SDS-PAGE.

Biological Activity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ug/ml can bind human TNFRSF1B, the EC50 is 1.632-2.699 ng/ml

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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