Recombinant Human TNF protein(active trimer), C-His Tag

Product Description

Cat

IMP-610

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(NP_000585.2)(Val 77-Leu 233), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 18.3 kDa. The protein migrates as 18.2 kDa under reducing (R) condition (SDS-PAGE).

Protein length

Val 77-Leu 233

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Human TNF-alpha, His Tag at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-13 ng/mL.

Bioactivity-SPR 1

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, His Tag (active trimer) (MALS verified) with an affinity constant of 0.244 nM as determined in a SPR assay.

Bioactivity-Cell based assay 1

Human TNF-alpha, His Tag (active trimer) (MALS verified) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.013-0.074 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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