Recombinant Human TNF protein(low endotoxin), C-His Tag

Product Description

Cat

IMP-616

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(NP_000585.2)(Leu 25-Ser 645), fused to C-Strep Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Ser 645

Endotoxin

Less than 0.01 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 6 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag, low endotoxin with a linear range of 0.8-6 ng/mL.

Bioactivity-SPR 1

Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R, His Tag, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay ( Routinely tested).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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