Recombinant Human TNF protein(primary amine labeling) (active trimer), Biotinylated

Product Description

Cat

IMP-609

Official Symbol

TNF

Product Overview

Biotinylated Recombinant Human TNF protein(NP_000585.2)(Val 77-Leu 233) was expressed in HEK293.

Expression System

HEK293

Species

Human

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.

Protein length

Val 77-Leu 233

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.
>95% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) with a linear range of 0.4-6 ng/mL.

Bioactivity-ELISA 2

Immobilized Human TNFR1, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) with a linear range of 0.4-6 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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