TNFa (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1β and IL-8 in cell supernatant will increase after stimulation. Therefore, a stimulation assay of TNFa was conducted using A549 cells. Briefly, A549 cells were incubated in DMEM with different concentrations of TNFa (1ng/mL, 10ng/mL, 100ng/mL, 1000ng/mL) for 8h, after which the concentration of IL-1β and IL-8 in the cell supernatant were detected by ELISA. IL-1β and IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β, the data was shown in Figure 1 and Figure 2 separately.

TNFa (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1β and IL-8 in cell supernatant will increase after stimulation. Therefore, a stimulation assay of TNFa was conducted using A549 cells. Briefly, A549 cells were incubated in DMEM with different concentrations of TNFa (1ng/mL, 10ng/mL, 100ng/mL, 1000ng/mL) for 8h, after which the concentration of IL-1β and IL-8 in the cell supernatant were detected by ELISA. IL-1β and IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β, the data was shown in Figure 1 and Figure 2 separately.