Recombinant Human TNFRSF10A Protein (Ala109-Asn239), C-hFc-tagged
Product Description
Cat
IMP-9872
Official Symbol
TNFRSF10A
Product Overview
Recombinant human TRAILR1/TNFRSF10A protein (Ala109-Asn239) with a Human IgG1 (Pro100-Lys330) at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Ala109
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 40.9 kDa (monomer)
SDS-PAGE: 50-55 kDa, reducing conditions
Protein length
Ala109-Asn239
Bio-activity
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.25-1.25 ng/mL in the presence of 6 ng/mL of Recombinant Human TRAIL/TNFSF10.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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