Recombinant Human TNFRSF10A Protein (Ala109-Asn239), C-hFc-tagged

Product Description

Cat

IMP-9872

Official Symbol

TNFRSF10A

Product Overview

Recombinant human TRAILR1/TNFRSF10A protein (Ala109-Asn239) with a Human IgG1 (Pro100-Lys330) at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.

Expression System

CHO

Species

Human

Tag

C-hFc

Predicted N Terminal

Ala109

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 40.9 kDa (monomer)
SDS-PAGE: 50-55 kDa, reducing conditions

Protein length

Ala109-Asn239

Bio-activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.25-1.25 ng/mL in the presence of 6 ng/mL of Recombinant Human TRAIL/TNFSF10.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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