Recombinant Human TNFRSF10B Protein (56-210aa), C-hIgG-His-Tagged
Product Description
Cat
IMP-9481
Official Symbol
TNFRSF10B
Product Overview
Recombinant human TNFRSF10B protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
TNFRSF10B, also known as tumor necrosis factor receptor superfamily member 10B isoform 1, is a member of receptors for the cytotoxic ligand TNFSF10/TRAIL. It can be activated by tumor necrosis factor-related apoptosis inducing ligand, and transduces an apoptosis signal. This protein promotes the activation of NF-kappa-B. Defects in this protein causes of head and neck squamous cell carcinomas.
Expression System
Baculovirus
Species
Human
Tag
C-hIgG-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
43.9 kDa (394 aa)
Protein length
56-210
AA Sequence
ITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPGTPASPCS<LEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤5 ng/mL with Human TRAIL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info