Recombinant Human TNFRSF14 protein, C-Fc Tag
Product Description
Cat
IMP-248
Official Symbol
TNFRSF14
Product Overview
Recombinant Human TNFRSF14 protein(AA Leu 39-Val 202)(Accession # NP_003811.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 43.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Leu 39-Val 202
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human HVEM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human HVEM, Fc Tag with a linear range of 0.3-20 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Human HVEM, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human BTLA (31-134), Fc,Avitag with a linear range of 0.156-5 ug/mL.

Bioactivity-BLI 1

Loaded Human HVEM, Fc Tag on Protein A Biosensor, can bind Human CD160, His Tag with an affinity constant of 0.44 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info