Human HVEM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human HVEM, Fc Tag with a linear range of 0.3-20 ng/mL.
Immobilized Human HVEM, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human BTLA (31-134), Fc,Avitag with a linear range of 0.156-5 ug/mL.
Loaded Human HVEM, Fc Tag on Protein A Biosensor, can bind Human CD160, His Tag with an affinity constant of 0.44 uM as determined in BLI assay (ForteBio Octet Red96e).