Recombinant Human TNFRSF14 Protein (Pro37-Val202, Ser108Thr, Ala140Arg), C-Fc and 6×His-tagged

Product Description

Cat

IMP-9772

Official Symbol

TNFRSF14

Product Overview

Recombinant human HVEM/TNFRSF14 protein, Pro37-Val202 (Ser108Thr; Ala140Arg), with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc and 6×His

Predicted N Terminal

Pro37

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 45 kDa (monomer)
SDS-PAGE: 60 kDa, reducing conditions

Protein length

Pro37-Val202, Ser108Thr, Ala140Arg

Bio-activity

Measured by its ability to inhibit TNF-beta -mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 2.5-10 μg/mL in the presence of 50 pg/mL of Recombinant Human TNF- beta /TNFSF1.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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