Recombinant Human TNFRSF14 Protein (Pro37-Val202, Ser108Thr, Ala140Arg), C-Fc and 6×His-tagged

Product Description

Cat

IMP-9772

Official Symbol

TNFRSF14

Product Overview

Recombinant human HVEM/TNFRSF14 protein, Pro37-Val202 (Ser108Thr; Ala140Arg), with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc and 6×His

Predicted N Terminal

Pro37

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 45 kDa (monomer)
SDS-PAGE: 60 kDa, reducing conditions

Protein length

Pro37-Val202, Ser108Thr, Ala140Arg

Bio-activity

Measured by its ability to inhibit TNF-beta -mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 2.5-10 μg/mL in the presence of 50 pg/mL of Recombinant Human TNF- beta /TNFSF1.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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