Recombinant Human TNFRSF18 protein, C-hFc Tag
Product Description
Cat
IMP-6030
Official Symbol
TNFRSF18
Product Overview
Recombinant Human TNFRSF18 protein(Q9Y5U5)(26-162aa), fused with C-terminal hFc tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-hFc Tag
Form
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Molecular Mass
40.8 kDa
Protein length
26-162aa
AA Sequence
QRPTGGPGCGPGRLLLGTGTDARCCRVHTTRCCRDYPGEECCSEWDCMCVQPEFHCGDPCCTTCRHHPCPPGQGVQSQGKFSFGFQCIDCASGTFSGGHEGHCKPWTDCTQFGFLTVFPGNKTHNAVCVPGSPPAEP
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 92% as determined by SDS-PAGE.
Biological Activity
①Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 μg/ml can bind TNFSF18, the EC50 is 2.565 to 2.940 ng/ml.②Human TNFRSF18 protein hFc tag captured on COOH chip can bind Human TNFSF18 protein hFc and Flag tag with an affinity constant of 38.5 nM as detected by LSPR Assay.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 ug/ml can bind TNFSF18, the EC50 is 2.565 to 2.940 ng/ml

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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