Recombinant Human TNFRSF18 Protein (Gln26-Glu161, Thr45Ala), C-hFc-tagged

Product Description

Cat

IMP-9868

Official Symbol

TNFRSF18

Product Overview

Recombinant human GITR/TNFRSF18 protein (Gln26-Glu161, Thr45Ala) with a Human IgG1 (Pro100-Lys330) at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc

Predicted N Terminal

No results obtained: Gln26 predicted

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 41 kDa (monomer)
SDS-PAGE: 50 kDa, reducing conditions

Protein length

Gln26-Glu161, Thr45Ala

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) GITR/TNFRSF18 Fc Chimera at 2 μg/mL (100 μL/well) can bind rhGITR Ligand with a linear range of 0.3-20 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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