Recombinant Human TNFRSF1A Protein, C-His&C-hFc-tagged
Product Description
Cat
IMP-4998
Official Symbol
TNFRSF1A
Product Overview
Recombinant human TNFRI (NP_001056.1) extracellular domain (Met 1-Thr 211) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Description
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.
Expression System
HEK293
Species
Human
Tag
C-His&C-hFc
Predicted N Terminal
Ile 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human TNFRI/Fc is a disulfide-linked homodimer. The reduced monomer consists of 438 amino acids and has a predicted molecular mass of 49.3 kDa. As a result of glycosylation, rh TNFRI/Fc monomer migrates with an apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Thr211
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured by its ability to inhibit TNF-α-mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 2-12ng/mL in the presence of 1 ng/mL recombinant human TNF-α.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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