Recombinant Human TNFRSF1A Protein (Leu30-Thr211), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10053
Official Symbol
TNFRSF1A
Product Overview
Biotinylated recombinant human TNF RI/TNFRSF1A protein (Leu30-Thr211) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Leu30
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 49 kDa
SDS-PAGE: 54-65 kDa, under reducing conditions.
Protein length
Leu30-Thr211
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human TNF alpha is coated at 0.100 μg/mL (100 μL/well), Biotinylated Recombinant Human TNF RI/TNFRSF1A Fc Chimera Avi-tag binds with an ED50 of 3.00-15.0 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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