Recombinant Human TNFRSF4 protein, C-mFc Tag

Product Description

Cat

IMP-014

Official Symbol

TNFRSF4

Product Overview

Recombinant Human TNFRSF4 protein(AA Leu 29-Ala 216)(Accession # P43489-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 47.1 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 29-Ala 216

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human OX40, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human OX40 Ligand, His Tag at 2 ug/mL (100 uL/well) can bind Human OX40, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.78-25 ng/mL.

Bioactivity-FACS 1

FACS assay shows that recombinant Human OX40, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.01 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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