Recombinant Human TNFRSF4 Protein (Leu29-Ala216), C-7×His and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10087
Official Symbol
TNFRSF4
Product Overview
Biotinylated recombinant human OX40/TNFRSF4 protein (Leu29-Ala216) with 7-His and Avi tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Expression System
CHO
Species
Human
Tag
C-7×His and Avi
Predicted N Terminal
Leu29
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 40-46 kDa, under reducing conditions
Protein length
Leu29-Ala216
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40 Ligand/TNFSF4 is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 His-tag Avi-tag binds with an ED50 of 150-900 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40 Ligand/TNFSF4 is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 His-tag Avi-tag binds with an ED50 of 150-900 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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