Recombinant Human TNFRSF4 Protein (Leu29-Ala216), C-7×His and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10087
Official Symbol
TNFRSF4
Product Overview
Biotinylated recombinant human OX40/TNFRSF4 protein (Leu29-Ala216) with 7-His and Avi tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Expression System
CHO
Species
Human
Tag
C-7×His and Avi
Predicted N Terminal
Leu29
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 40-46 kDa, under reducing conditions
Protein length
Leu29-Ala216
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40 Ligand/TNFSF4 is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 His-tag Avi-tag binds with an ED50 of 150-900 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40 Ligand/TNFSF4 is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 His-tag Avi-tag binds with an ED50 of 150-900 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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