Recombinant Human TNFRSF4 Protein (Leu29-Ala216), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10016

Official Symbol

TNFRSF4

Product Overview

Alexa Fluor 488 conjugated recombinant human OX40/TNFRSF4 Fc protein (Leu29-Ala216) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Leu29

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 46.7 kDa (monomer)
SDS-PAGE: 70 kDa, under reducing conditions.

Protein length

Leu29-Ala216

Bio-activity

Measured by flow cytometry for its ability to bind anti-Human OX40/TNFRSF4 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 15.0-150 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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