Recombinant Human TNFRSF4 Protein (Leu29-Ala216), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10016

Official Symbol

TNFRSF4

Product Overview

Alexa Fluor 488 conjugated recombinant human OX40/TNFRSF4 Fc protein (Leu29-Ala216) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Leu29

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 46.7 kDa (monomer)
SDS-PAGE: 70 kDa, under reducing conditions.

Protein length

Leu29-Ala216

Bio-activity

Measured by flow cytometry for its ability to bind anti-Human OX40/TNFRSF4 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human OX40/TNFRSF4 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 15.0-150 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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