Recombinant Human TNFRSF8 Protein, C-hFc&AVI-tagged, Biotinylated
Product Description
Cat
IMP-1976
Official Symbol
TNFRSF8
Product Overview
Recombinant human TNFRSF8 (NP_001234.2) (Met1-Lys379) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. CD30 protein is expressed by activated, but not resting, T and B cells. CD30 can regulate proliferation of lymphocytes and may also play an important role in human immunodeficiency virus replication. As a regulator of apoptosis, CD30 protein induces cell death or proliferation, depending on the cell type, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD30 protein expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs), and CD30 is also linked to leukocytes in patients with chronic inflammatory diseases, including lupus erythematosus, asthma, rheumatoid arthritis and atopic dermatitis (AD).
Expression System
HEK293
Species
Human
Tag
C-hFc&AVI
Predicted N Terminal
Phe19
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human TNFRSF8 consists of 614 amino acids and predicts a molecular mass of 67.02 kDa. It migrates as an approximately 110.33 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Lys379
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human CD30L hFc at 2 μg/mL (100 μL/well) can bind Human CD30/TNFRSF8 hFc&AVI, Biotinylated, the EC50 of Human CD30/TNFRSF8 hFc&AVI, Biotinylated is 30-130 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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