Recombinant Human TNFRSF8 Protein (Phe19-Lys379), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10010

Official Symbol

TNFRSF8

Product Overview

Alexa Fluor 488 conjugated recombinant human CD30/TNFRSF8 Fc protein (Phe19-Lys379) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in human embryonic kidney cell.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Expression System

HEK293

Species

Human

Tag

C-Fc

Predicted N Terminal

Phe19

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 65 kDa
SDS-PAGE: 80-110 kDa, under reducing conditions.

Protein length

Phe19-Lys379

Bio-activity

Measured by flow cytometry for its ability to bind HEK293 human embryonic kidney cells transfected with human CD30 Ligand. The concentration of Recombinant Human CD30/TNFRSF8 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 3.00-30.0 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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