Recombinant Human TNFRSF8 Protein (Phe19-Lys379)
Product Description
Cat
IMP-9853
Official Symbol
TNFRSF8
Product Overview
Recombinant human CD30/TNFRSF8 protein (Phe19-Lys379) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Expression System
Mouse myeloma cell line
Species
Human
Tag
Tag Free
Predicted N Terminal
Phe19
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 38.4 kDa (monomer)
SDS-PAGE: 65-85 kDa, reducing conditions
Protein length
Phe19-Lys379
Bio-activity
Measured by its ability to block CD30 Ligand-induced IL-6 secretion by HDLM human Hodgkin's lymphoma cells. The ED50 for this effect is 1.00-5.00 μg/mL in the presence of 50 ng/mL of Recombinant Human CD30 Ligand.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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