Recombinant Human TNFRSF9 protein, C-Fc Tag

Product Description

Cat

IMP-270

Official Symbol

TNFRSF9

Product Overview

Recombinant Human TNFRSF9 protein(AA Leu 24-Gln 186)(Accession # NP_001552.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 43.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 24-Gln 186

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human 4-1BB, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human 4-1BB, Fc Tag at 0.1 ug/mL (100 uL/well) can bind Biotinylated Human 4‑1BB Ligand, Fc Tag, Avi Tag with a linear range of 0.4-13 ng/mL.

Bioactivity-ELISA 2

Immobilized Human 4-1BB, Fc Tag at 1 ug/mL (100 uL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.2-3 ng/mL.

Bioactivity-BLI 1

Loaded Human 4-1BB, Fc Tag on Protein A Biosensor, can bind Human 4-1BB Ligand, His Tag with an affinity constant of 1.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human 4-1BB, Fc Tag can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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