Recombinant Human TNFRSF9 protein, C-His Tag

Product Description

Cat

IMP-135

Official Symbol

TNFRSF9

Product Overview

Recombinant Human TNFRSF9 protein(AA Leu 24-Gln 186)(Accession # Q07011-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.2 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 24-Gln 186

Endotoxin

Less than 0.01 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human 4‑1BB, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human 4-1BB Ligand (71-254), Fc Tag, active trimer at 1 ug/mL (100 uL/well) can bind Human 4-1BB, His Tag, low endotoxin with a linear range of 0.2-3 ng/mL.

Bioactivity-ELISA 2

Immobilized Human 4-1BB, His Tag, low endotoxin at 1 ug/mL (100 uL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.1-3 ng/mL.

Bioactivity-BLI 1

Loaded Human 4-1BB Ligand (71-254), Fc Tag, active trimer on Protein A Biosensor, can bind Human 4‑1BB, His Tag, low endotoxin with an affinity constant of 10.6 nM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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