Recombinant Human TNFRSF9 protein, C-mFc Tag

Product Description

Cat

IMP-157

Official Symbol

TNFRSF9

Product Overview

Recombinant Human TNFRSF9 protein(AA Leu 24-Gln 186)(Accession # Q07011-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 44.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 24-Gln 186

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human 4‑1BB Ligand, Fc Tag at 1 ug/mL (100 uL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.6-5 ng/mL.

Bioactivity-ELISA 2

Immobilized Human 4‑1BB Ligand, His Tag at 2 ug/mL (100 uL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.1-4 ng/mL.

Bioactivity-ELISA 3

Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin at 1 ug/mL (100 uL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.2-6 ng/mL.

Bioactivity-FACS 1

Flow Cytometry assay shows that Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.03 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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