Recombinant Human TNFRSF9 Protein (Leu24-Gln186), C-hFc and 6×His-tagged
Product Description
Cat
IMP-9916
Official Symbol
TNFRSF9
Product Overview
Recombinant human 4-1BB/TNFRSF9 protein (Leu24-Gln186) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc and 6×His
Predicted N Terminal
Leu24
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 44.8 kDa (monomer)
SDS-PAGE: 50-65 kDa, reducing conditions
Protein length
Leu24-Gln186
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant human 4-1BB Fc Chimera is Immobilized at 10 ng/mL (100 μL/well), the concentration of recombinant human 4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info