Recombinant Human TNFSF10 protein, No tag

Product Description

Cat

IMP-1252

Official Symbol

TNFSF10

Product Overview

Recombinant Human TNFSF10(P50591)(Arg115-Gly281) protein, fused with no tag, was expressed from E.coli.

Expression System

E.coli

Species

Human

Tag

No tag

Form

Lyophilized from sterile 20mM PB, 250mM Nacl, pH7.5.

Molecular Mass

The protein has a calculated MW of 19.5kDa, and migrates as 16kDa under reducing conditions.

Protein length

Arg115-Gly281

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2.5-15 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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