Recombinant Human TNFSF10 protein, No tag

Product Description

Cat

IMP-1252

Official Symbol

TNFSF10

Product Overview

Recombinant Human TNFSF10(P50591)(Arg115-Gly281) protein, fused with no tag, was expressed from E.coli.

Expression System

E.coli

Species

Human

Tag

No tag

Form

Lyophilized from sterile 20mM PB, 250mM Nacl, pH7.5.

Molecular Mass

The protein has a calculated MW of 19.5kDa, and migrates as 16kDa under reducing conditions.

Protein length

Arg115-Gly281

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2.5-15 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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