Recombinant Human TNFSF10 Protein, Tag free
Product Description
Cat
IMP-9112
Official Symbol
TNFSF10
Product Overview
Human TNFSF10 (P50591, 114 a.a.-281 a.a ) partial recombinant protein expressed in E. coli.
Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
Expression System
E. coli
Species
Human
Tag
Tag free
Form
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 μg/mL.
Molecular Mass
19.6 kDa
Protein length
114-281
AA Sequence
MVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG
Endotoxin
< 0.2 EU/μg of protein by gel clotting method
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.
Applications
Functional Study; SDS-PAGE
Storage
Store at 4 to 8 centigrade for 1 week. For long term storage store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Bioactivity-ELISA 1
Result of activity analysis

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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