Recombinant Human TNFSF11(active trimer) protein, N-His Tag

Product Description

Cat

IMP-625

Official Symbol

TNFSF11

Product Overview

Recombinant Human TNFSF11 protein(NP_003692.1)(Gly 64-Asp 245), fused to N-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 22.4 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gly 64-Asp 245

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (active trimer) with a linear range of 0.4-6 ng/mL.

Bioactivity-ELISA 2

Immobilized Human OPG, Fc Tag at 2 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (active trimer) with a linear range of 0.2-2 ng/mL.

Bioactivity-BLI 1

Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0.409 nM as determined in BLI assay.

Bioactivity-SPR 1

Anti-TNFSF11 (human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0.274 nM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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