Recombinant Human TNFSF13 Protein (Ala105-Leu250), N-HA-tagged
Product Description
Cat
IMP-9844
Official Symbol
TNFSF13
Product Overview
Recombinant human APRIL/TNFSF13 protein (Ala105-Leu250) with a HA (YPYDVPDYA) tag at N-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13.
Expression System
HEK293
Species
Human
Tag
N-HA
Predicted N Terminal
Tyr
Form
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 21.7 kDa
SDS-PAGE: 26 kDa, reducing conditions
Protein length
Ala105-Leu250
Bio-activity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 5-25 ng/mL in the presence of goat anti-mouse IgM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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