Recombinant Human TNFSF13B Protein (Ala134-Leu285), N-10×His-tagged

Product Description

Cat

IMP-9718

Official Symbol

TNFSF13B

Product Overview

Recombinant human BAFF/BLyS/TNFSF13B protein (Ala134-Leu285) with MHHHHHHHHHH tag at N-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Expression System

Mouse myeloma cell line

Species

Human

Tag

N-10×His

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 19.6 kDa
SDS-PAGE: 22-29 kDa, reducing conditions

Protein length

Ala134-Leu285

Bio-activity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.4-2 ng/mL in the presence of goat anti-mouse IgM μ chain.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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