Recombinant Human TNFSF13B Protein (Ala134-Leu285), N-10×His-tagged

Product Description

Cat

IMP-9718

Official Symbol

TNFSF13B

Product Overview

Recombinant human BAFF/BLyS/TNFSF13B protein (Ala134-Leu285) with MHHHHHHHHHH tag at N-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Expression System

Mouse myeloma cell line

Species

Human

Tag

N-10×His

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 19.6 kDa
SDS-PAGE: 22-29 kDa, reducing conditions

Protein length

Ala134-Leu285

Bio-activity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.4-2 ng/mL in the presence of goat anti-mouse IgM μ chain.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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