Recombinant Human TNFSF14 protein, N-His Tag

Product Description

Cat

IMP-140

Official Symbol

TNFSF14

Product Overview

Recombinant Human TNFSF14 protein(AA Asp 74-Val 240)(Accession # O43557-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the N-terminus.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from sterile PBS with Arginine, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Asp 74-Val 240

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human LIGHT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human HVEM, Fc Tag at 2 ug/mL (100 uL/well) can bind Human LIGHT, His Tag with a linear range of 1-16 ng/mL.

Bioactivity-BLI 1

Loaded Human DcR3, Fc Tag on AHC Biosensor, can bind Human LIGHT, His Tag with an affinity constant of 4.94 nM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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