IMP-387

TNFSF14

Recombinant Human TNFSF14 protein (NP_001363816.1) (Asp74-Val240) was expressed in HEK293, fused with the Fc region of rabbit IgG at the N-terminus..

TNFSF14, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / TNFSF14 / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / TNFSF14 / CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of TNFSF14 in vivo. TNFSF14 / TNFSF14 / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4 and CD T cells. In addition, TNFSF14 / TNFSF14 / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / TNFSF14 / CD258 is being actively investigated as a possible basis for cancer treatment.

HEK293

Human

N-rFc Tag

Ser

Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

The recombinant human TNFSF14 consists of 418 amino acids and predicts a molecular mass of 45.7 kDa.

Asp74-Val240

< 1.0 EU per μg protein as determined by the LAL method.

> 90 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.