Recombinant Human TNFSF18 Protein (Glu52-Ser177), N-HA-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10019

Official Symbol

TNFSF18

Product Overview

Alexa Fluor 488 conjugated recombinant human GITR Ligand protein (Glu52-Ser177) at N-terminus with a HA (YPYDVPDYA) tag was expressed in Chinese Hamster Ovary cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.

Expression System

CHO

Species

Human

Tag

N-HA

Predicted N Terminal

Tyr

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 19.6 kDa (monomer)
SDS-PAGE: 20-27 kDa, under reducing conditions.

Protein length

Glu52-Ser177

Bio-activity

Measured by flow cytometry for its ability to bind anti-human GITR Ligand/TNFSF18 antibody conjugated beads. The concentration of Recombinant Human GITR Ligand/TNFSF18 (GCN4-IZ) Alexa Fluor 488 that produces 50% of the binding response is 3.00-30.0 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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