Recombinant Human TNFSF18 Protein (Glu52-Ser177), N-HA-tagged

Product Description

Cat

IMP-9874

Official Symbol

TNFSF18

Product Overview

Recombinant human GITR Ligand/TNFSF18 protein (Glu52-Ser177) with a Hemagglutinin Tag (YPYDVPDYA) at N-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.

Expression System

CHO

Species

Human

Tag

N-HA

Predicted N Terminal

Tyr

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 19.6 kDa (monomer)
SDS-PAGE: 21-30 kDa, reducing conditions

Protein length

Glu52-Ser177

Bio-activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human GITR. The ED50 for this effect is 4-20 ng/mL. The activity can be enhanced approximately 10-fold in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Non-covalently-linked trimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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