Recombinant Human TNFSF18 Protein (Glu52-Ser177), N-HA-tagged
Product Description
Cat
IMP-9874
Official Symbol
TNFSF18
Product Overview
Recombinant human GITR Ligand/TNFSF18 protein (Glu52-Ser177) with a Hemagglutinin Tag (YPYDVPDYA) at N-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.
Expression System
CHO
Species
Human
Tag
N-HA
Predicted N Terminal
Tyr
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 19.6 kDa (monomer)
SDS-PAGE: 21-30 kDa, reducing conditions
Protein length
Glu52-Ser177
Bio-activity
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human GITR. The ED50 for this effect is 4-20 ng/mL. The activity can be enhanced approximately 10-fold in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Non-covalently-linked trimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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