Recombinant Human TNFSF18 Protein (Glu74-Ser199), N-6×His-tagged

Product Description

Cat

IMP-9873

Official Symbol

TNFSF18

Product Overview

Recombinant human GITR Ligand/TNFSF18 protein (Glu74-Ser199) with an N-terminal 6×His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus) cells.

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.

Expression System

Sf 21 cells

Species

Human

Tag

N-6×His

Predicted N Terminal

His

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 15 kDa
SDS-PAGE: 14 kDa-16 kDa, reducing conditions

Protein length

Glu74-Ser199

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human GITR/TNFRSF18 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human GITR Ligand/TNFSF18 binds with an ED50 of 30-300 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry